Isolation of salt tolerant endophytic and rhizospheric bacteria by natural selection and screening for promising plant growthpromoting rhizobacteria pgpr and. Genes that could increase salt tolerance fall into three main functional groups. A salttolerant microorganism staphylococcus xylosus was isolated from a vegetable pickled plant containing about 7. Pdf potential use of beneficial salt tolerant bacteria for.
Salt tolerant plant growth promoting rhizstephan obacteria for enhancing crop productivity of saline soils article pdf available in frontiers in microbiology december 2019 with 321 reads. Growth, sporulation, and germination of clostridium perfringens in media of controlled water activity. Red pepper seedlings inoculated with acc deaminasecontaining salt tolerant bacteria reduced 57% stress ethylene production and produced similar. Potential use of beneficial salt tolerant bacteria for.
One loop of each bacterial culture was streaked onto nfree agar containing 0. An alternative strategy to improve crop salt tolerance may be to introduce salt tolerant microbes. In this study, 305 bacteria were isolated from paddy soil in taoyuan, china. Plant growthpromoting rhizobacteria enhance salinity. Alleviation of salt stress by halotolerant and halophilic plant growth. The isolates were tolerant to 48% nacl in nb medium and 34% nacl concentration in nitrogen free nfb medium. Endophytic bacteria in plant salt stress tolerance. Full length research paper osmoadaptation and plant growth. Developing salt tolerant crops has been a much desired scientific goal but with little success to date, as few majordeterminant genetic traits of salt tolerance have been identified flowers, 2004.
Allam et al potential use of beneficial salt tolerant bacteria for improving wheat productivity grown in salinized soil germinating ability by 2030% because of the production of the plant growthpromoting compounds 25. Biocompost is prepared by composting pressmud a sugar industry byproduct received from cane juice filtration and spent wash received from. Isolation, bioprospecting and diversity of salt tolerant bacteria associated with sugarcane in soils of pernambuco, brazil1 maria c. Sl12 isolated from a salt lake, and to evaluate its capability to promote growth in wheat plants triticum. In biotechnology, such salt tolerant bacteria are widely used for the production of valuable enzymes, and more than a thousand years ago humans began using salt. Greenway department ofagronomy, institute ofagriculture, university ofwestern australia, nedlands 6009, australia c. Halophilic bacteria are organisms which thrive in salt rich environments, such as salt lakes, solar salterns and salt mines which contain large populations of these organisms. Among these, 162 strains were tested for salt tolerance. When droplets evaporate, the salt s sodium and chlorine ions can penetrate stems, buds and leaves, causing direct damage.
Isolation and characterization of high salt tolerant bacteria from agricultural soil jaymin mendpara 1, vivek parekh 1, sudhir vaghela 1, atul makasana 1, prashant d. In the present study, salt tolerant rhizobacteria were isolated from the roots of a. Salttolerant and plant growthpromoting bacteria isolated from zn. Proline over production results enhanced osmotolerance in s. Halomonas is a genus of halophilic salt tolerating proteobacteria. The first approach was used to assess the presence of salt tolerant bacteria and the relationships among salt nacl resistance phenotype, soil characteristics and phylogenetic assignment of strains. Biological aerobic treatment of saline wastewater provides the material of this study. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Soil salinity is a major abiotic stress that limits plant growth, and inoculating plant growth. Probiotic potential of lactic acid bacteria isolated from. Key words salt, salt tolerance, planting guide, roadside plants 18.
They grow in the presence of oxygen, although some. Pdf wheat is one of the major crops cultivated in the black clay soil, the salinized soil reduces the. Among the strains examined the potassium contents varied about fivefold and were positively. This study shows that inoculation of paddy oryza sativa with such bacteria could provide salttolerant ability by reducing the toxicity of reactive oxygen species by reducing plant cell membrane. Holotolerant and holophilic microorganisms, in microbiology of extreme environments. Halophiles are organisms that live in highly saline environments, and require the salinity to survive, while halotolerant organisms belonging to different domains. Uzbek microbiologist dilfuza egamberdieva hopes to apply her new agricultural technique soon in uzbekistan to boost the yield of economically important crops. Knowing how salt differentially affects organisms is particularly valuable for people who study extremophiles. Pdf salt tolerant plant growth promoting rhizstephan. Isolation, bioprospecting and diversity of salttolerant. Pdf endophytic bacteria in plant salt stress tolerance. Various genera of salt tolerant plant growth promoting rhizobacteria stpgpr have been isolated from extreme alkaline, saline, and sodic soils.
We describe the effect of salt stress on the bacterial biofilm formation and eps accumulation at varying salt concentrations. Salttolerant plant growthpromoting bacteria enhanced salinity. However, some salt tolerant bacteria improve the health of plants under saline stress. A salt tolerant microorganism staphylococcus xylosus was isolated from a vegetable pickled plant containing. Total community analysis, performed by trflp on total dna, was carried out to investigate the relationships between total community. Isolation and characterization of high salt tolerant.
Filippova 19578 found that some strains of root module bacteria of lucerne were salt resistant. The presentation of the authors names and or special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript. Halomonas elongata, a new genus and species of extremely. The aim of this study was to characterize a salt tolerant pgp bacterium serratia sp. Comparative study of wild and transformed salt tolerant. In the current study, seventeen lactic acid bacteria lab and probiotic strains were screened for survival in simulated gastric juice ph 3 and 2 and bile 0. Current and future prospects article pdf available in journal of plant growth regulation november 2018 with 1,769 reads how we measure reads. Osmond research school ofbiological sciences, australian national university, canberra 2601, australia abstract. The tolerant bacteria at 8% wv nacl were selected and.
This combination of morphology, salt tolerance, and guanine plus cytosine content supports the establishment of a new genus, halomonas, in family ii vibrionaceae of part 8, gramnegative facultatively anaerobic bacteria, of bergeys manual 8th edition. Kunjadia 2, gaurav sanghvi 3, devendra vaishnav 3 and gaurav s. The sodium and potassium content non halophilic bacteria in relation to salt tolerance. The role of salt tolerant bacteria in plant growth promotion makes them ideal candidates for improving crop yield nasim et al. Induced salt tolerance of perennial ryegrass by a novel. Isolation and characterization of high salt tolerant bacteria from agricultural soil. Salttolerant and plant growthpromoting bacteria isolated.
Eleven salt tolerant bacteria isolated from different sources soil, plants and their transformed strains were used to study their influence on triticum aestivum var. Unfortunately, this book cant be printed from the openbook. Halotolerant rhizobacteria promote growth and enhance. Characterization of a salttolerant and coldadapted. The coldadapted enzymes are most active at low and medium temperatures, which has obvious advantages in the bioprocess, such as saving energy, reducing production costs, and. Salt stress tolerant genes in halophilic and halotolerant bacteria. The selection of salinity tolerant bacteria may provide greater promotion of plant growth in soils with salt stress. Molecular diversity and enzymatic potential of salt. Isolation of salt tolerant endophytic and rhizospheric bacteria by natural selection and screening for promising plant growthpromoting rhizobacteria pgpr and growth vigour in tomato under sodic environment. Moreover, it may increase the crop productivity of coastal saline lands in bangladesh by transferring the salt tolerant genes into the plants.
The bacteria had indole3acetic acids iaa production, nitrogen fixation, and phosphate solubilization, under 8% wv nacl condition. Salt responses of enzymes from species differing in salt. Salttolerant and plantgrowthpromoting bacteria isolated. The salt tolerant plants are divided into lists by salt tolerance, plant characteristics, and groups associated by light and moisture requirements.
A close relative of thoroughly explored glycophytic crucifer a. The bacteria of pdmcd0501, pdmcd2007, and pdmzncd2003 were isolated from a zncd contaminated soil. The salt tolerance study was carried out by spreading 0. Sodic soils have immense productivity potential, if managed through proper technology interventions. If you need to print pages from this book, we recommend downloading it as a pdf. Candidate genes for salt tolerance and results of transformation experiments. Selection, identification and characterization of the microorganism were carried out. Trees and shrubs that tolerate saline soils and salt spray. Download pdf files of individual articles andor entire issues.
As a study organism we used heterotrophic bacteria, serratia marcescens from american type culture collection strain atcc 880, that is a gramnegative, rod shaped bacteria that do not form spores. Biological treatment of tannery wastewater by using salt. Pdf isolation and characterization of high salt tolerant bacteria. Isolation, bioprospecting and diversity of salttolerant bacteria. The salt tolerance of each strain was assessed by determining the lowest water activity at which growth occurred in sodium chloride medium. Commercial lactic acid bacteria and probiotic strains. Pdf biological treatment of saline wastewater using a. The sodium and potassium content of nonhalophilic bacteria in relation to salt tolerance. Selecting and planting salt tolerant trees and shrubs. Keywords azotobacter, salinity, wheat, salt tolerant, bacteria. The effects of salt concentration on bacterial growth. Sample is serially diluted and then plated out on an agar surface in such a manner that single isolated bacteria form visible isolated colonies, the number of colonies can be used as a measure of the number of viable living cells in that known dilution. Dgge was carried out with five different primer sets targeting 16s rrna gene of salttolerant alkaliphilic actinomycetes isolated from coastal gujarat western india 22. Salt tolerance and dependence are the characteristics of some microbes.
Salt spray the aerial drift of salt laden water droplets that are deposited on trees and shrubs causes salt spray damage. Salt tolerant xylanases can be applied in marine products, wastewater processing, and bioethanol production from seaweeds bai et al. Tolerance of bacteria to high concentrations of nacl and. Isolation of salt tolerant strains of rhizobium trifolii 327 the presence of high sodium chloride has been reported to reduce the number of rhizobia in legume inoculants garg et al. They were classified as salt tolerant bacteria in this experiment. Halotolerance is the adaptation of living organisms to conditions of high salinity.
Microbial amelioration of crop salinity stress journal. They were classified as salttolerant bacteria in this experiment. The type species of this genus is halomonas elongata. Isolation and characterization of halotolerant bacteria and its effects. This manual is a tool for ridot landscape and engineering personnel to help select plants for roadside use in rhode island. The accepted manuscript in pdf format is listed with the files at the bottom of this page. Number of bacteria in a given sample is usually too great to be counted directly. Halotolerant species tend to live in areas such as hypersaline lakes, coastal dunes, saline deserts, salt marshes, and inland salt seas and springs. There are some mesophilic bacteria that are salt tolerant. The sodium and potassium contents of cells of 32 strains of nonhalophilic bacteria were determined after growth in a standard medium of low salt content. Identifying of novel halophilic and halotolerant bacteria that promote plant growth can be used as alternatives for.
When it comes to growing bacteria in a laboratory, scientists provide six basics to ensure cell growth. Salttolerant plant growthpromoting bacteria enhanced salinity tolerance of salt tolerant alfalfa medicago sativa l. Members of halomonas are gramnegative, rodshaped bacteria, generally 0. Isolation and identification of halotolerant soil bacteria from coastal. Salt tolerant microbes can survive in osmotic and ionic stress. Pdf isolation and characterization of salt tolerant. Increased salt tolerance of perennial species used for fodder or fuel production is a key component in reducing the spread of secondary salinity, while increased salt tolerance of crops will directly improve production in soils with primary salinity appendix s1. Salt tolerant bacterial mixed consortia showed appreciable biodegradation at all saline concentrations 2%, 4%, 6%, 8% and 10% wv with 80% cod reduction in particular at 8% salinity level the consortia could be used as suitable working cultures for tannery saline wastewater treatment. Salt is an important nutrient for growing bacteria in culture because it allows scientists to select for or against halophilic organisms.
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